case report Serological and Molecular Identification of Rare Type B (A): A Case Report
Authors: Ziyi He1*,Yingming Hu1, Xianguo Xu2, Siping Cui1
*Corresponding Author: Ziyi He, Department of Research Transfusion, Dongguan Blood Center, No.3 TianXin Road, Dongguan 523000, P.R. China
1Blood Transfusion Laboratory, Dongguan Blood Center, P.R. China
2Institute of Blood Transfusion, Zhejiang Blood Center, P.R. China
Received Date: 28 January, 2022
Accepted Date: 02 February, 2022
Published Date: 07 February 2022
Citation: He Z, Hu Y, Xu X, Cui S (2022) Serological and Molecular Identification of Rare Type B (A): A Case Report. Ann Case Report 7: 766. DOI: https://doi.org/10.29011/2574-7754.100766
Abstract
Objective: To investigate the characteristics of one case of rare B (A) blood group was identified by serology test and DNA sequencing. Methods: ABO blood phenotype were determined by routine serological methods and ABO genotype was determined by direct sequencing. Results: ABO group identification is inconsistent with the forward and reverse typing, an antigen is relatively weak, the forward typing is A2B or AwB, and the reverse typing shows the existence of weak anti-A1, H antigen and O phenotype of reaction agglutination intensity is consistent. Forward and reverse direct gene sequencing on exons 6 and 7 of the ABO gene with the O 02 allele in exon 6 and a unique single base 700C/G mutation in exon 7 confirmed that the patient's genotype was ABO*BA.02/O02. The serological phenotype of the patient was determined to be B (A). Conclusion: Blood group serology identified A2B or AWB, which should be further characterized by B(A) typing and molecular analysis, the case was identified as A rare ABO*BA.02/O 02.
Keywords: B (A) blood type; ABO genotype; Gene sequencing; Molecular