Article / Research Article

"Forensic Analysis of a Confiscated Illicit Heroin Sample"

Farid A. Badria*, Mona El-Neketi, Hassan-Elrady A. Saad

Department of Pharmacognosy, Mansoura University, Mansoura, Egypt

*Corresponding author: Farid A. Badria, Department of Pharmacognosy, Mansoura University, Mansoura 35516, Egypt. Tel: +201001762927; Email: faridbadria@gmail.com

Received Date: 17 February, 2018; Accepted Date: 02 March, 2018; Published Date: 09 March, 2018

1.       Abstract

Different forensic analytical methods involving physical and microscopical examination, color tests, Thin Layer Chromatography (TLC) and Gas Chromatography/ Mass Spectrometry (GC/MS) were applied for the identification and characterization of an illicit heroin sample confiscated in Egypt. These methods confirmed that the heroin sample under investigation is a poorly manufactured sample, prepared by acetylation with acetic anhydride, badly stored, adulterated and it is of the South West Asian type and suggested to be abused by inhalation through smoking.

2.       Keywords: Chromatography-Mass Spectrometry (GC-MS); Confiscated illicit; Heroin; opium alkaloids; TLC 

1.       Introduction

Heroin (Diacetylmorphine) is a semi-synthetic analogue of morphine, prepared by acetylating with acetyl chloride or acetic anhydride. It belongs to the internationally controlled narcotic analgesics with morphine, codeine and other synthetic drugs [1,2]. It was some seventy years after the first isolation of morphine from opium before the synthesis of diacetylmorphine was first reported in 1874. Commercial production by the Bayer Company, who named this new drug heroin, began in 1898. By the beginning of the twentieth century, heroin was widely accepted by the medical profession, and was typically used as a substitute for codeine and morphine in tuberculosis and other respiratory diseases. It was also about this time that heroin first appeared in China. A few years after the 1925 International Convention on Narcotics, international controls began to limit the supply of heroin, and the clandestine manufacture of heroin began [1-3]. No one could have suspected this would become one of the most notorious drugs of our time. The global illicit production of opium (from which heroin is processed) becomes increasingly concentrated in Afghanistan which has been producing three-quarters of the world's illicit opium [4] 

Analysis of illicit heroin samples in criminal cases to identify their main active principles, diluents, adulterants and impurities that may adversely affect the abuser’s heath is important for judicial purposes, identification of the source of a sample, tracing of distribution routes and identifying new production processes and ascertains whether two or more exhibits came from an identical source [3,5]. Different forensic analytical methods are concerned with heroin analysis these methods include: presumptive color tests3, [6-10]; Thin Layer Chromatography [3,7,11-19]. Without question the gas chromatography interfaced Mass Spectrometer (GC/MS) is one of the most useful tools available to the forensic drug chemist. It is able to provide highly specific spectral data on individual compounds in a complex mixture of compounds, without prior separation of these components [3]. Many GC/MS methods were published for illicit heroin preparations analysis [20-27]. In this report, the analyses of heroin, its impurities and adulterants using color and precipitation tests, TLC, GC/MS are described with the aim of providing information about the origin, the trafficking and synthetic routes of illicit heroin samples.

2.        Experimental

2.1    Material and Reagent

                2.1.1           Drug 

 Confiscated heroin sample: It was obtained from seizure number 978 / 1988, Suez- Egypt.                 

                2.1.2           Reference Materials 

Opiate alkaloids

·         Morphine sulfate ampoules (20 mg/ml) from Misr Co. for Pharmaceutical industry, Egypt.

·         Codeine phosphate powder from Supreme organization of Drugs and Medical Requirements, Cairo (Czechoslovakia).

·         Papaverine hydrochloride powder from BDH chemical, Ltd. England

·         Noscapine: It was isolated from Noscapine SyrupÒ (Agropharm, Buckingham House, church Road, Pann, Bucks. HP10 8LN).

·         Heroin (diacetylmorphine) and O3-Monoacetylmorphine were prepared in our laboratory from morphine.

·         O6-monoacetylmorphine hydrochloride: United Nations UNIES Vienna international center. Chief, scientific section, DOA/ UNDDP, Howard Street.

·         Acetylcodeine: It was prepared in our laboratory from codeine.

The identity of the prepared compounds was confirmed by comparison of their spectral data (UV, IR and EI-MS) with the previously published ones [2,3,7,10,26,28-30]

2.2    Apparatus 

·         Leitz Wetzlar microscope GMBH fitted with camera Lucida (Germany).

·         Precoated silica gel G60F254 (20 x 20 cm x 0.2 mm thick) on aluminum (E-Merck and Machery-Nagel, Germany), Ultra-violet lamp operating at λ 254, 366 nm (Desaga, Germany) for location of TLC spots, Glass jars of different sizes, micro-pipettes for spotting.

·         GC/MS was Carried out at National Research Center, Dokki, Cairo, Egypt on GC/MS Finnigan mat SSQ7000 chromatograph with Digital DEC 3000 Work station. Helium was used as carrier gas at a flow rate of 1.6 ml/min and column head pressure was 13 Psi. The gas chromatograph was coupled with mass detector (MS) at 70 eV in EI ionization mode               

2.3    Chemicals and Reagents 

The following reagents were used during the course of this work: 

·         Derivatizing reagents: N-methyl-Trimethyl Silyltrifluoro acetamide (MSTFA) reagent for silylation was purchased from Sigma USA.

·         All reagents and solvents for TLC separation were of analytical grade and purchased from (Adwik, Egypt), while those for GC/MS analysis were of spectroscopic grades.

·         Color reagents: Marquis, Froehde, Meck,s reagent, Chromatographic Spray reagents: Dragendroff,s and acidified Potassium iodoplatinnate were prepared according to United Nations publications [3]. 

2.4    General Procedure

                2.4.1           Microscopic Characters, Color, Solubility and Precipitation (Anion) Tests

                2.4.1.1      Sample Preparation

·         Microscopic characters: About 0.5 g of the heroin sample was dissolved by gentle shaking in 10 ml of distilled water. The obtained solution was centrifuged, the clear supernatant was separated, transferred to a clean test tube and reserved for carrying out the color tests (solution 1), and the residue was subjected to exhaustive washing with water to be suitable for microscopic examination [3]. Several mounts of vegetable debris were prepared in water, chloral hydrate, and phloroglucinol and conc. HCl and examined under the microscope.

        ·         For color tests: The previously reserved clear supernatant of the heroin sample was used (solution 1). Other portions of heroin were dissolved in methanol [1mg in 0.5ml] for Oliver test8 and [10 mg in 1ml] for Murexide test31 then centrifuged. With a disposable pipette, the supernatant was drawn into a clean test tube (solution 2&3).

        ·         For solubility test: About 100 mg of illicit heroin sample was dissolved in 0.5ml distilled water (to test its solubility in water). The same steps were repeated but water was replaced by ethanol (to test the presence of carbohydrates) [2,7].

        ·         For precipitation (Anion) test: About 1g of the illicit heroin sample was dissolved in approximately 5ml distilled water and centrifuged. The supernatant was removed to a clean test tube (solution 4) for precipitation test [2,3,7].

                2.4.1.2      Color and Precipitation (Anion) Tests Procedures

The procedures for presumptive color and precipitation (Anion) tests are cited in (Table 1).

2.4.2           Thin Layer Chromatography (TLC)

                2.4.2.1      Developing solvents

System A: Chloroform-n-hexane-triethylamine (9:9:4 v/v)16

System B: Chloroform-Methanol (9:1 v/v)7

                2.4.2.2      Sample and standard solutions preparation

Five mgs heroin sample were dissolved in 1 ml methanol and centrifuged. The supernatant was separated into a clean vial, from which 3µl were spotted to the TLC plate. Morphine sulfate, codeine phosphate, papaverine hydrochloride, O6-mono-acetylmorphine hydrochloride, acetyl codeine, heroin and caffeine were made at conc. 5 mg / ml methanol. Noscapine was made at the same concentration but in chloroform [3].

                2.4.2.3      TLC separation

The methanolic solution of heroin sample and standard solutions were examined by TLC on pre-coated silica gel plates using solvent systems A and B. The developed plates were air dried and visualized under UV at 254 nm followed by spraying separately with Dragendroff,s and acidified potassium iodoplatinnate reagents.

                2.4.3           Gas Chromatography Interfaced Mass Spectrometer (GC/MS)             

                2.4.3.1      Sample Preparatio 

Illicit heroin sample was subjected to several sample preparations either as total or neutral fraction for full sample characterizatio 

A)     Total illicit heroin sample

1) Direct analysis: Five mgs of illicit heroin sample were dissolved in 1 ml methanol- chloroform (4:1 v/v), sonicated for 10 min. and 2 µl of this solution were injected into HP-5 column.

2) Analysis after derivatization: Five mgs of illicit heroin sample were subjected to silylation with 150 µl of N-methyl N-Tri Methyl Silyl Tri Fluro-Acetamide (MSTFA) in 1.2 ml chloroform- pyridine (5:1 v/v) for 10 minutes at 70 °C. After 1 hour at room temperature, 2µl of this mixture were injected into HP-5 column and 1µl into DB-1 column.

         B)      Neutral fraction 

For the determination of the neutral components of the illicit heroin sample, 30 mgs of illicit heroin sample were dissolved in 10 ml of 0.5 N sulfuric acid, extracted by shaking with ether (2x, 5 ml each), and centrifuged. The ethereal layer was dried over anhydrous sodium sulfate, evaporated to dryness using a rotary evaporator, the residue was dissolved in 75 µl of chloroform and 2µl were injected directly into HP-5 column [7,27,32]. Silylation of the neutral fraction is not recommended as reported by [32,27]. The identification of the components was achieved by comparing the fragmentation pattern of the resulting mass spectra with those recorded in mass library spectral database and published data.

                2.4.3.2      Operating conditions for GC [2,3,7,33](Table 1b)

3.       Results and discussion

3.1    Results

                3.1.1           Physical Characters

The physical characters of illicit heroin sample are summarized in (Table 2).

                3.1.2           Microscopic Examination

The following fragments (Figure 2) were detected

1-Abundant starch granules: simple, small rounded granules, with centric split or cleft hilum and faint concentric striations which are more visible in larger granules and measuring 11-12-15 μ. (D.).

2-Long fibers: with thin non-lignified wall, wide lumen and acute apex measuring 225-456-825 μ. (L.) and 15-24-30 μ. (W.)

3-Short fibers: with thick non-lignified wall, narrow lumen and bluntly pointed apex measuring 122-211-237 μ. (L.) and 17-20-23 μ. (W.).

                3.1.3           Color, Solubility and Precipitation Tests

The results are shown in (Table 3)

                3.1.4           TLC

The results are shown in (Table 4)

                3.1.5           GC/MS

The GC chromatograms are shown in (Figures 3-6), while the results of GC/MS are cited in (Table 5,6) and illustrated in (Figures 7). The identified components of the heroin sample are listed in (Table 7) and shown in (Figures 8).

4.       Discussion

From the physical characters of illicit heroin, one may conclude the following:

1. It is crudely processed heroin sample as indicated by its dark brown or black color [43].

2. Its strong vinegar-like odor suggests the use of excess acetic anhydride as an acetylating agent or hydrolysis of heroin due to bad storage in humid atmosphere [2,3,7].

3. Microscopic examination: of the sample revealed the presence of abundant starch granules suggested that the heroin sample under investigation was adulterated or diluted with starch [12]. Color, Solubility and Precipitation (Anion) tests: From the data cited in (Table 3), one could conclude the following:

·         Presumptive color tests indicated the presence of opium alkaloids, heroin and caffeine which is commonly used as heroin adulterant.

·         Solubility and precipitation tests showed that the heroin sample is present in the sulfate salt form diluted with starch which is insoluble in water and ethanol [l].

·         The TLC data cited in (Table 4), revealed the presence of diacetylmorphine (heroin), O6-monoacetylmorphine (O6-MAM), Acetylcodeine (AC), morphine, codeine, noscapine, papaverine and caffeine in the heroin sample under investigation. The presence of caffeine in the heroin sample could indicate that it is of the South West Asian type characterized by its cutting with caffeine [3,7].

4.1    GC/MS

GC/MS investigation of total heroin sample as well as neutral fraction revealed the presence of natural impurities from opium (24 components), impurities from the manufacturing process (5 components) and added adulterants (5 components). The identified components and their mean relative percentage are shown in (Table 7) and the structures of the identified components are illustrated in (Figure 8). From the fore mentioned data, one may conclude the following:

1.Heroin is present in a relatively low concentration (0.6%) and O6-monoacetyl-morphine (O6- MAM) is the dominant component (34.4%) which could be attributed to the following:

·         The use of excess H2SO4 during the manufacture process of heroin that result in the hydrolysis of heroin to yield high percentage of O6-MAM and morphine (> 5%). These features are characteristics for poorly manufactured heroin which is the case of our sample [2].

·         Partial hydrolysis of heroin to O6-MAM and then to morphine upon storage under humid condition [2,24,27] and the liberation of acetic acid2 which is responsible for the strong vinegar-like odor of our sample.

·         Partial hydrolysis of heroin to O6-MAM during injection into the GC system and/or during the silylation process [27].

2. O3-monoacetylmorphine (O3-MAM) was not detected in our sample, as it is the product resulting from incomplete acetylation of morphine [2,24]. The absence of O3-MAM and the presence of high percentage O6-MAM (>10% relative to heroin) and morphine (>1% relative to heroin) confirms that post-processing hydrolysis have occurred to our sample [27].

3. Thebaine was never detected in our heroin sample as it is unstable towards acetylation conditions. However, 3,6 Dimethoxy- 4,5 epoxyphenanthrene was detected as its decomposition product resulting from the reaction of thebaine with acetic anhydride [3,27,38].

4. Noscapine concentration in the sample was found to be about 13.7% (< 46%), which means that the present noscapine is a natural impurity from opium and is a non-intentionally added adulterant [21]. N-Acetylanhydronornarceine and N- Acetyl-nornarcotine were detected as the decomposition products of noscapine resulting from its reaction with acetic anhydride only or with acetic anhydride and oxygen, respectively [27].

5. The absence of 1-chloroheroin and 3-[1-(1-carboxymethoxyethyl)]-6-acetylmorphine (the 2 route specific markers for acetyl chloride and ethylene diacetate) as components in the confiscated heroin sample excludes the use of acetyl chloride and ethylene diacetate as acetylating agents and support the use of acetic anhydride as an acetylating agent in the manufacturing process (3-United Nations, 1998; 5-Odell et al., 2006).

6.The lipid fraction consisting of fatty acid and fatty acid methyl esters was well resolved only on DB-1 column due to the low polarity of DB-1 in comparison to HP-5. The presence of this lipid fraction is characteristic for low quality crude morphine produced in South West Asia [33].

7. It is important to note that, the fatty acid should be present in the original sample as volatile derivative with undetectable molecular ion peaks.

8. Polar compounds like morphine, monoacetylmorphine and Acetylcodeine were well resolved on polar stationary Phases HP-5 [44].

9. Caffeine and barbiturates (Phenobarbital and methyl phenobarbital) are characteristic as adulterants for South West Asian heroin. Caffeine was added to the heroin sample to enhance the amount of vaporized heroin without decomposition and improve the taste [45], while barbiturates were added as hypnotics [46].

Therefore, it could be concluded that the illicit heroin sample under investigation is a poorly manufactured sample, badly stored, adulterated and it is of the south West Asian type.

5.       Conclusion

Application of physical and microscopical examination, color tests, Thin Layer Chromatography (TLC) and Gas Chromatography/ Mass Spectrometry (GC/MS) proved to be a very valuable tool for the characterization of the heroin sample under investigation, which has the following characteristics:

a.        Physical characters: dark brown to nearly black, small granular pieces (chunks) having strong vinegar-like odor.

b.       The absence of O3-MAM and the presence of high percentage O6-MAM content (>10% relative to heroin) and morphine (>1% relative to heroin) confirms that post-processing hydrolysis have occurred to our sample.

c.        The presence of heroin, morphine and O6-MAM at a concentration of about 37.0%; Noscapine (~ 13.7 %), papaverine (~ 2.2%) and Acetylcodeine (~7.9%) is characteristic for SWA type.

d.       The relatively high concentration of noscapine (13.7%) relative to morphine (5%) confirms that the heroin sample is prepared via method 3 in which noscapine and morphine are in the ration 2:1 characteristic for crude morphine of SWA.

e.        The presence of natural impurities such as meconine, noscapine, papaverine, cryptopine, laudanosine, fatty acids and fatty acid methyl esters confirms that it is a crudely processed heroin.

f.        The presence of heroin as the sulfate salt.

g.       The presence of abundant starch granules as adulterant.

h.       The presence of caffeine and barbiturates (Phenobarbital and methylpheno-barbital) as adulterants is characteristic for SWA type.

Therefore, we can conclude that the heroin sample under investigation is: a poorly manufactured sample, prepared by acetylation with acetic anhydride, badly stored, adulterated, of the South West Asian type and suggested to be abused by inhalation through smoking.


       

Confiscated heroin (Mona-1)

Confiscated heroin (Mona-2)

heroin-13A

A

B

C

A. Original Package

B. Cylindrical piece

C. Heroin chunks


Figure 1: Confiscated heroin (seizure number 978/1988, Suez-Egypt).




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Figure 2: Vegetable debris of the heroin sample.





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Figure 3: GC of the non silylated heroin sample on (HP-5) column.


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Figure 4: GC of the silylated heroin sample (MSTAF) on (HP-5) column.


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Figure 5: GC of the silylated heroin sample (MSTAF) on (DB-1) column.



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Figure 6: GC of non silylated neutral fraction of the heroin sample using HP-5 column.




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Figure 7: EI-MS spectrum of the detected components in heroin samples.


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Figure (7): Cont.

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Figure (7): Cont.


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Figure 7: Cont.


Heroin



Natural impurities from opium

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Figure 8: Identified components of the illicit heroin sample.

Impurities from manufacture process

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Adulterants

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Figure 8: Cont.




Test Name

Method

Use

Ref

Presumptive color tests:

Marquis, Froehde, Meck,s and nitric acid test

One drop of heroin solution (solution 1) was placed in each well in a spot tile followed by adding 3 drops of each Marquis, Froehde, Meck,s and nitric acid reagent.

Heroin and opium alkaloid detection

[2,3,6-8]

Oliver test:

A trace of copper sulfate solution (1% in water) was added by means of a glass rod wetted with copper sulfate to solution 2. The solution was stirred, and 1ml of 3% hydrogen peroxide and 1ml of conc. ammonium hydroxide were added and shaken.

Heroin detection

[6,8]

Murexide test

One ml of 10% HCl was mixed with 1.0 ml of solution 3 to which 0.1g. Potassium chlorate was added in white porcelain dish and evaporated to dryness on a water bath. The residue was exposed separately to amm. vapor.

Caffeine detection

[31]

Test for anions

Silver nitrate test

A portion of solution 4 was added to few drops of silver nitrate 5.0% w/v solution.

[2]

Chloride precipitate is insoluble in conc. Nitric, soluble in dilute ammonia solution, from which it can be precipitated by addition of nitric acid.

Chloride detection

Tartarate precipitate is soluble in nitric acid.

Tartarate detection

Citrate precipitate is soluble in nitric acid.

Citrate detection

Acetic anhydride test

A small amount of illicit heroin sample was heated with 0.5 ml acetic anhydride at 80 °C for 10 minutes.

Citrate detection

Barium chloride test

Another portion of solution 4 was added to few drops of barium chloride 10% w/v solution. Sulfate precipitate is insoluble in HCl.

Sulfate anion detection

[2,3]

 

Table 1a: Procedures for presumptive color and precipitation tests of the heroin sample.

 

Column (HP-5)

Fused silica capillary column

(5% phenyl) Methylsiloxane (HP-5)

(0.25) I.D, 30 m. length

Volume of injected sample

2 μl

Injector temperature

250 °C

Column temperature

Start at 150 °C, Increase at 6 °C/ minute to 280 °C, isothermal for 10 minute, Increase at 9 °C/ minute to 300 °C, isothermal for 15 minute, end of program

Column (DB-1)

Fused silica capillary column

Dimethyl-polysiloxane (DB-1)

(0.32) I.D, 30 m. length

Volume of injected sample

1 μl

Injector temperature

250 °C

Column temperature

Starts at 150 °C, Increases by 9 °C/ minute to 300 °C, isothermal for 10 minute, end of program


Seizure shape

Cylindrical pieces with rounded ends, wrapped externally with green adhesive tape and internally with another wrapping of yellowish-white to light brown paper from which the sample was obtained (chunks) measuring 0.5-1.5 cm D. Figure (1 A&B)

Sample shape

Small granular pieces (chunks) of about 0.5 to 1.5 cm in diameter (Figure 1 C)

Sample weight

50 g

Sample color

Dark brown to nearly black.

Sample touch

Rough

Sample odor

Strong vinegar- like odor.

5-Solubility

Soluble in water producing brownish turbid solution containing vegetable debris.

 

Table 2: Physical characters of illicit heroin sample.

Test Name

Result

Indication

Presumptive color tests

Marquis

A violet / reddish purple color

Presence of morphine, codeine or heroin.

Froehde

A purple / green color

Presence of morphine, codeine or heroin.

Meck

A blue / green color

Presence of morphine, codeine or heroin.

Nitric acid

A red-orange color was obtained at first which was gradually changed to a bright green color

Presence of heroin.

Oliver

A persistent pink to red color

Presence of heroin

Murexide

Purple color after exposure to ammonia vapor.

Presence of caffeine or other xanthine alkaloids

Solubility tests

Solubility in water

Soluble in water leaving vegetable debris

Presence of heroin salt

Solubility in ethanol

Soluble in ethanol, leaving vegetable residue

Presence of starch

Precipitation (Anion) test

Silver nitrate

No white precipitate

Absence of chloride ion.

Barium chloride

White precipitate

Presence of sulfate ion.

 

Table 3: Results of the presumptive color and precipitation tests for the heroin sample.

The results are shown in (Table 4)

 

Reference

Rf x100

Heroin sample

System A

System B

Morphine

16.9

14.5

(+)

Codeine

36.9

28.9

(+)

Caffeine

40.9

72.6

(+)

O6- monoacetylmorphine

66.15

27.6

(+)

Papaverine

68.7

78.9

(+)

Heroin

69.2

63.16

(+)

Acetylcodeine

75.8

55.3

(+)

Noscapine

91.0

89.5

(+)

 

Table 4: Results of TLC investigation of the heroin sample.

 

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Table 5: Results of GC-MS of identified components of the heroin sample before and after silylation (MSTAF) using HP-5 and DB-1 columns.

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Table (5): Cont.

 

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Table 6: Results of GC-MS of identified components of non silylated neutral fraction of the heroin sample using HP-5 column.

 

Component

Molecular formula

Molecular weight

Mean relative %

Notes

Heroin

C21H23NO5

369

0.6

 

Natural impurities from opium:

Hydrocotarnine

C12H15NO3

221

1.94

 

Codeine

C18H21NO3

299

3.6

 

Morphine

C17H19NO3

285

5

 

Papaverine

C20H21NO4

339

2.2

 

Cryptopine

C21H23NO5

369

0.07

 

Noscapine

C22H23NO7

413

13.7

 

laudanosine

C21H27NO4

357

Trace

 

Meconine

C10H10O4

194

5.7

 

Methyl palmitate (Hexadecanoic acid methyl ester)

C17H34O2

270

3.4

 

Palmitic acid (Hexadecanoic acid)

C16H32O2

256

Trace

 

Methyl oleate  (9-Octadecenoic acid methyl ester)

C19H36O2

296

2.5

 

Methyl stearate (Octadecanoic acid methyl ester)

C19H38O2

298

0.8

 

n-Tricosane

C23H48

324

Trace

 

3,6 Dimethoxy- 4,5 epoxy-phenanthrene

C16H12 O3

252

0.8

Thebaine + Ac2O

Decomposition product

n- Tetracosane

C24H50

338

Trace

 

Oleoamide (9-Octadecenamide)

C18H35NO

281

Trace

 

Stearamide (Octadecanamide)

C18H37NO

283

Trace

 

Pentacosane

C22H52

352

Trace

 

Hexacosane

C26H54

366

Trace

 

Octacosane

C28H58

394

Trace

 

Squalene

C30H50

410

Trace

 

Nonacosane

C29H60

408

Trace

 

Triacontane

C30H62

422

Trace

 

Hentriacontane

C31H64

436

Trace

 

Impurities from manufacturing process:

O6 - Monoacetylmorphine

C19H21NO4

327

34.4

Present in high concentration due to hydrolysis of heroin

 

(Small quantities from morphine + Ac2O)

Acetylcodeine

C20H23NO4

341

7.9

 

N- Acetylnorlaudanosine

C22H27NO5

234

0.06

 

N- Acetylnornarcotine

C23H23NO8

441

Trace

Noscapine + O2 + Ac2O Decomposition product

N-Acetylanhydronornarceine

C24H25NO8

455

Traces

Noscapine + Ac2O Decomposition product

Adulterants:

Nicotinamide

C6H6N2O

122

1.39

 

Diphenylamine

C12H11N

169

Trace

 

 

Table 7: Identified components of the illicit heroin sample.

 

 

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Citation: Badria FA, El-Neketi M, Saad HA (2018) Forensic Analysis of a Confiscated Illicit Heroin Sample. Curr Res Bioorg Org Chem: CRBOC-105. DOI: 10.29011/CRBOC -105. 100005