A sensitive, selective and precise high performance thin layer chromatographic method has been developed for the estimation of chlorzoxazone, paracetamol, diclofenac potassium and famotidine in the pharmaceutical dosage form. TLC aluminum plates pre-coated with silica gel 60F254 used as the stationary phase, while chloroform: methanol: ethyl acetate: hexane: ammonia (10: 2.5: 1.5: 1: 0.1, v/v/v/v/v) used as mobile phase. The Rf values were observed 0.74 ± 0.01, 0.52 ± 0.01, 0.30 ± 0.01 and 0.14 ± 0.01 for chlorzoxazone, paracetamol, diclofenac potassium and famotidine, respectively. The densitometry analysis was carried out in absorbance mode at 282 nm. The method was linear in the range of 250-1500 ng/spot for chlorzoxazone, diclofenac potassium and famotidine and 500- 3000 ng/spot for paracetamol and method was validated as per ICH guideline. The limit of detection and limit of quantization were found to be 35.98 ng/spot and 109.05 ng/spot, respectively for chlorzoxazone, 99.74 ng/spot and 302.25 ng/spot, respectively for paracetamol, 58.63 ng/spot and 177.69 ng/spot, respectively for diclofenac, and 50.93 ng/spot and 154.35 ng/spot, respectively for famotidine. The proposed method was successfully applied to the estimation of chlorzoxazone, paracetamol, diclofenac potassium and famotidine in the pharmaceutical dosage form.
Keywords: Chlorzoxazone Paracetamol; Chlorzoxazone (CLZ); Paracetamol (PCM); Diclofenac potassium (DCL); Famotidine (FAM); HPTLC; Validation; Chlorzoxazone Dosage