"Litsea glutinosa potentiates the M3-muscarinic acetylcholine receptor to normalize diarrheal incidences"

Md Atiar Rahman

University of Chittagong, Bangladesh

DOI: 10.29011/2574-7711-C1-002

Diarrhea is one of the leading causes of preventable death in developing countries which mainly affects children under five. This research identified the novel compounds of Litsea glutinosa and investigated its antidiarrheal potential in microorganism- and chemical-induced diarrheal model. M3 muscarinic acetylcholine and 5-HT3 targets were undertaken to justify the binding affinity of plant products using molecular docking analysis. A pure compound oxoaporphine was identified from its methanol extract. In microorganism induced diarrhea, methanol extract showed the highest zone of inhibition 24 ± 0.9 against Shigella dysentreae and 16.00 ± 1.14 against E. coli which were statistically significant compared to Ciprofloxacin. In castor oil induced diarrhea, same extract inhibited 82.50% of diarrhea which was significant compared to loperamide. Similarly, methanol extract significantly reduced the average number of diarrheal feces in magnesium sulfate induced diarrhea. In gastrointestinal motility study, methanol extract in most of the cases except in Castor oil induced model showed the lowest peristalsis index. In biochemical assay, ethyl acetate and methanol extract restored the Na+, K+, Cl- and HCO3- levels in induced diarrheal model. Ethyl acetate extract significantly managed to normalize the serum IgE and C-reactive protein. Serum triglyceride and creatinine levels were also restored by methanol extract. The sample was found to be nontoxic in acute toxicity test. The oxoaporphine showed lower binding energy which is close to loperamide. The results demonstrate a strong support for therapeutic use of this plant in diarrheal evidences with further conformation in a dose response study of standardized extract.

Md. Atiar Rahman, has been working as a Principal Investigator of Laboratory of Alternative Medicine and professor and chair of the Department of Biochemistry and Molecular Biology, University of Chittagong, Bangladesh. He has started his journey after completing PhD from Ehime University, Japan and he published more than 80 articles in peer reviewed high impact journals and contributing as editorial board member of some prestigious journals. He has presented his works in more than 16 countries. He is representing number of relevant prestigious societies. His interest is to explore the novel therapeutic options and functional food for chronic diseases.